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Title: Genetic markers for signalling and diagnosis of sexual disruption in roach (Rutilus rutilus)
Author: Environment Agency
Document Type: Monograph
Annotation: Environment Agency Project ID:EAPRJOUT_1125, Representation ID: 360, Object ID: 2389
The work described supports an Environment Agency project funded to Exeter that has been investigating the ontogeny of sexual development and effects of long-term exposure to oestrogenic chemicals on sexual development and function in the roach, Rutilus rutilus. The report provides a brief overview of the extensive genetic and genomic resources that have been developed for studies into sexual function in the roach. These include the cloning of 42 genes in the roach, quantitative assays to measure expression of individual genes (qRT-PCR assays) and the establishment of gene arrays (allowing for studies into the expression of thousands of roach genes simultaneously). The report also describes how the molecular tool box developed has been applied to investigate the molecular mechanisms controlling sexual development in male and female roach and used to investigate the mechanisms of action of environmental oestrogens. In the results some of the findings are presented on the normal ontogeny of expression of oestrogen receptors and aromatase genes that are known to play central roles in reproduction. In the oestrogen exposure studies, data are presented that illustrate altered patterns in the expression of specific genes can signal (and potentially provide early warnings) for sexual disruption by environmental oestrogens. The gene array work illustrates how this approach can provide deeper insights into the modes of action of environmental oestrogens and biological processes that are subsequently disrupted.
Publisher: Environment Agency
Subject Keywords: Freshwater fishesCoarse fishesSexual reproductionGeneticsMolecular biologyOestrogensFreshwater ecology
Taxonomic Keywords: Rutilus rutilus
Extent: 34
Total file downloads: 241

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