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Title: Development of a field test kit for detection of blue-green algal toxins (348/01/A) : interim progress report
Author: National Rivers Authority
Document Type: Monograph
Work has commenced on Stage 1 of this project to develop monoclonal antibodies to microcystin LR (research work commenced on the 8th November 1991). To date three immunisation conjugates of microcystin LR have been prepared (3a, 3b and 4c) along with two microcystin LR conjugates (4a and b) for coating o f ELISA plates. Balb C and Balb CX C57B1 (FI) hybrid mice have received three inoculations and serum samples obtained for antibody quantification. Initial analysis of the circulating antibodies by ELISA show that antibodies against microcystin-LR are present, albeit at low titres. Difficulty has been experienced at obtaining sufficient microcystin LR for immunisation and ELISA plate coating purposes.
Publisher: National Rivers Authority
Publication Date: 1992
Publication Place: Bristol
Subject Keywords: CyanophyceaeLaboratory experimentationAntibodiesImmunityBacterial counting
Extent: 14
Total file downloads: 21

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